Standard curve
Example of IL-13 standard curve in Assay Diluent .
Mouse IL-13 Single Plex Assay (适用于Luminex)
Mouse IL-13 Single Plex Assay (适用于Luminex)
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Product Details
Product Details
Product Specification
| Antigen | IL-13 |
| Reactivity | Mouse |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: ＜10%； Inter-assay: ＜20% |
| Sample type | Serum; Plasma; Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | ＜2pg/mL |
| Range | 0.26pg/mL-50000pg/mL |
| Recovery | Plasma: 80%-120% Serum: 80%-120% Cell culture supernatant: 80%-120% |
| Assay time | 70 minutes |
| Species reactivity | Mouse |
Background
Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. The secondary structural features of IL-13 are similar to that of Interleukin 4 (IL-4); however it only has 25% sequence identity to IL-4 and is capable of IL-4 independent signaling. IL-13 is a cytokine secreted by T helper type 2 (Th2) cells, CD4 cells, natural killer T cell, mast cells, basophils, eosinophils and nuocytes. Interleukin-13 is a central regulator in IgE synthesis, goblet cell hyperplasia, mucus hypersecretion, airway hyperresponsiveness, fibrosis and chitinase up-regulation. It is a mediator of allergic inflammation and different diseases including asthma.
Picture
Picture
Luminex
Quantifiction of Mouse spleen cells
Stimulated sample: Mouse spleen cells were stimulated with 4 ug/mL ConA for 24h, and the average concentration of IL-13 in the supernatant was measured to be 211.99 pg/mL.
Unstimulated sample: Mouse spleen cells were cultured for 24h, and the average concentration of IL-13 in the supernatant was measured to be 1.35 pg/mL.
Protocol Diagram
