Standard curve
Example of Mouse IL-13 standard curve in Assay Diluent #12Y.
Mouse IL-13 OneStep ELISA kits
Mouse IL-13 OneStep ELISA kits
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Product Details
Product Details
Product Specification
| Antigen | IL-13 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 3.4%； Inter-assay: 4.9% |
| Sample type | Cell culture supernatant, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 2.413 pg/mL |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Recovery | Cell culture supernatant: 108% Plasma: 98% |
| Assay time | 60 minutes |
| Species reactivity | Ms |
Background
Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. The secondary structural features of IL-13 are similar to that of Interleukin 4 (IL-4); however it only has 25% sequence identity to IL-4 and is capable of IL-4 independent signaling. IL-13 is a cytokine secreted by T helper type 2 (Th2) cells, CD4 cells, natural killer T cell, mast cells, basophils, eosinophils and nuocytes. Interleukin-13 is a central regulator in IgE synthesis, goblet cell hyperplasia, mucus hypersecretion, airway hyperresponsiveness, fibrosis and chitinase up-regulation. It is a mediator of allergic inflammation and different diseases including asthma.
Picture
Picture
ELISA
Linearity
The concentrations of Mouse IL-13 were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: EL-4 cells stimulated with 10ug/ml PHA and 10ug/ml PMA for 2days (20%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 5348.63 pg/mL in stimulated EL-4 cells supernatant.
To assess the linearity of the assay, the samples was spiked with high concentrations of Mouse IL-13 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
To assess the linearity of the assay, the samples was spiked with high concentrations of Mouse IL-13 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Protocol Diagram
