Standard curve
Example of Mouse IL-12 P70 standard curve in Assay Diluent EA2.
Mouse IL-12 P70 Serum OneStep ELISA Kit
Mouse IL-12 P70 Serum OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IL-12 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 2.6%； Inter-assay:2.9% |
| Sample type | Serum |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 5.205 pg/mL |
| Range | 7.81 pg/mL – 500 pg/mL |
| Recovery | Serum: 99% |
| Assay time | 60 minutes |
| Species reactivity | Ms |
Background
Mouse IL-12 is a critical pro-inflammatory cytokine that bridges innate and adaptive immunity. It is a heterodimeric protein composed of p35 (IL-12A) and p40 (IL-12B) subunits, forming the bioactive IL-12(p70). Primarily produced by antigen-presenting cells like dendritic cells and macrophages upon pathogenic stimulation, its pivotal role is to drive the differentiation of naïve CD4⁺ T cells into T helper 1 (Th1) cells and to potently induce interferon-gamma (IFN-γ) production from both T cells and natural killer (NK) cells. This establishes a robust cellular immune response essential for defense against intracellular pathogens such as viruses and bacteria. In biomedical research, Mouse IL-12 is a cornerstone for studying Th1-mediated immunity, evaluating vaccine efficacy, investigating antitumor immunotherapy strategies, and modeling autoimmune diseases like experimental autoimmune encephalomyelitis (EAE).
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ELISA
Linearity
The concentrations of Mouse IL-12 were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: CD-1 Mouse serum. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 14.72 pg/mL in CD-1 Mouse serum.
#2 sample is undiluted samples are as follows: BALB/c Mouse serum. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 12.82 pg/mL in BALB/c Mouse serum.
#3 sample is undiluted samples are as follows: C57BL/6 Mouse serum. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 36.29 pg/mL in C57BL/6 Mouse serum.
To assess the linearity of the assay, sample was spiked with high concentrations of Mouse IL-12 P70 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Protocol Diagram
