Immobilized Mouse IL-1 beta/IL-1F2 Protein, His tag at 2 μg/mL (100 μL/well) can bind Human IL-1R2/CD121b Protein, hFc tag (Cat. No. S0A1129) with EC50 of 10.5-15.3 ng/ml.
Mouse IL-1 beta/IL-1F2 Protein, His tag
Mouse IL-1 beta/IL-1F2 Protein, His tag
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$250.00 USD
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | Interleukin-1 beta, IL-1 beta, Il1b |
| Accession | P10749 |
| Amino Acid Sequence | Protein sequence (P10749, Val118-Ser269, with N-His tag) VPIRQLHYRLRDEQQKSLVLSDPYELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS |
| Expression System | HEK293 |
| Molecular Weight | Predicted MW: 19.6 kDa Observed MW: 20.0 kDa |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | with N-His tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4. |
| Reconstitution | Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation. |
| Stability & Storage | 12 months from date of receipt, -20 to -70 °C as supplied. |
Background
IL-1β is a member of the interleukin 1 family of cytokines. This cytokine is produced by activated macrophages, monocytes, and a subset of dendritic cells known as slanDC, as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. IL-1β, in combination with IL-23, induced expression of IL-17, IL-21 and IL-22 by γδ T cells. Increased production of IL-1β causes a number of different autoinflammatory syndromes, most notably the monogenic conditions referred to as Cryopyrin-Associated Periodic Syndromes (CAPS). Intestinal dysbiosis has been observed to induce osteomyelitis through a IL-1β dependent manner.
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Bioactivity
SDS-PAGE
2μg（R: reducing conditions）
