Standard curve
Example of Mouse IgG2c standard curve in Assay Diluent #1.
Mouse IgG2c Onestep ELISA Kit
Mouse IgG2c Onestep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IgG2c |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 3.2%； Inter-assay: 7.2% |
| Sample type | Cell culture supernatant; Serum; Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 3.418 pg/mL |
| Range | 39.06 pg/mL – 2500 pg/mL |
| Recovery | Cell culture supernatant: 89% Serum: 83% Plasma: 97% |
| Assay time | 60 minutes |
| Species reactivity | Ms |
Background
Mouse IgG2c is the functional equivalent and primary Th1-associated antibody isotype in C57BL/6 and related mouse strains, replacing the conventional IgG2a found in other strains like BALB/c. This expression is due to a natural allelic variation in the Igh-1 locus. Functionally, IgG2c mirrors IgG2a by exhibiting high affinity for activating Fc gamma receptors, making it exceptionally potent at eliciting effector mechanisms such as antibody-dependent cellular cytotoxicity (ADCC), phagocytosis (ADCP), and complement-dependent cytotoxicity (CDC). Consequently, it is a critical mediator of protective immunity against intracellular pathogens and a key indicator of a robust Th1-type immune response in these specific mouse strains.
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ELISA
Linearity
The concentrations of mouse IgG2c were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: C57BL/C mouse serum was 200,000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 403.69 ug/mL in C57BL/C mouse serum.
#2 sample is undiluted samples are as follows: CD-1 mouse serum was 200,000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 77.99 ug/mL in CD-1 mouse serum.
Linearity
The concentrations of mouse IgG2c were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: C57BL/C mouse plasma (Heparin) was 200,000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 388.92 ug/mL in C57BL/C mouse plasma (Heparin).
#2 sample is undiluted samples are as follows: C57BL/C mouse plasma (EDTA) was 200,000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 187.04 ug/mL in C57BL/C mouse plasma (EDTA).
#3 sample is undiluted samples are as follows: C57BL/C mouse plasma (Sodium citrate) was 200,000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 105.68 ug/mL in C57BL/C mouse plasma (Sodium citrate).
Specificity
Detection of mouse IgG2c in serum from different mouse strains.
Protocol Diagram
