Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cells) labeled with Mouse IgG2a, κ Isotype Control antibody at 1/20 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Mouse IgG2a (D265A) isotype control (S-5115)
Mouse IgG2a (D265A) isotype control (S-5115)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Clone Number | S-5115 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM, ICFCM |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
Picture
Picture
FC
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cells) fixed with 4% paraformaldehyde and permeabilized with 0.1% Tween. Cells were labeled with Mouse IgG2a, κ Isotype Control antibody at 1/20 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
