Standard curve
Example of mouse IgG (Total) standard curve in Assay Diluent #3.
Mouse IgG (Total) OneStep ELISA Kit
Mouse IgG (Total) OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IgG (Total) |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision |
Intra-assay: 4.4%； Inter-assay: 6.1% |
| Sample type | Cell culture supernatant, Serum, Plasma, Cell/Tissue Lysate |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 16 pg/ml |
| Range | 78 pg/ml - 5000 pg/ml |
| Recovery | Cell culture supernatant 104% Serum 107% Plasma 103% |
| Assay time | 60 minutes |
| Species reactivity | Mouse |
| CROSS REACTIVITY | / |
| INTERFERENCE | / |
| NIBSC convert | / |
Background
Immunoglobulin G (IgG) is a type of antibody. IgG is the most common type of antibody found in blood circulation. IgG molecules are created and released by plasma B cells. In mice, the IgG class is further divided into four subclasses: IgG1, IgG2a/ IgG2c (strain specific), IgG2b, and IgG3. The mean serum Ig levels found for 6-month-old B6 mice were 0.22 mg/ml for IgM, 0.28 mg/ml for IgG1, 1.22 mg/ml for IgG2b, 0.18 mg/ml for IgG3, 0.075 mg/ml for IgA and about 0.7 mg/ml for IgG2a. This kit is for the quantitative measurement of mouse total IgG in mouse serum, plasma and supernatant from cell cultures.
Picture
Picture
ELISA
Demonstration of the tested species specificity
Linearity
The concentrations of total IgG were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Mouse serum was 10^6 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3mg/mL in mouse serum.
#2 sample is undiluted samples are as follows: Mouse plasma was 10^6 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 2.2 mg/mL in mouse plasma.
Leading Competitor comparison
Protocol Diagram
Linearity
The concentrations of Mouse IgG were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Mouse spleen Lysate was 400fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3661.17 ng/mL in stimulated Mouse spleen Lysate.
#2 sample is undiluted samples are as follows: Mouse placenta Lysate was 400fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1332.27 ng/mL in stimulated Mouse placenta Lysate.
#3 sample is undiluted samples are as follows: Mouse liver Lysate was 400fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 183.70 ng/mL in stimulated Mouse liver Lysate.
#4 sample is undiluted samples are as follows: Mouse lung Lysate was 400fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 2537.59 ng/mL in stimulated Mouse lung Lysate.
#5 sample is undiluted samples are as follows: Mouse muscle Lysate was 400fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 2035.67 ng/mL in stimulated Mouse muscle Lysate.
#6 sample is undiluted samples are as follows: Mouse heart Lysate was 400fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 2002.79 ng/mL in stimulated Mouse heart Lysate.
#7 sample is undiluted samples are as follows: Mouse brain Lysate was 400fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 435.37 ng/mL in stimulated Mouse brain Lysate.
