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Mouse IgG (Total) Broad range ELISA Kit

Mouse IgG (Total) Broad range ELISA Kit

Catalog Number: S0C3038 Application: ELISA Reactivity: Mouse Conjugation: Brand: Starter
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Regular price $500.00 USD
Regular price Sale price $500.00 USD
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Product Details

Product Specification


Antigen IgG (Total)
Immunogen Recombinant Protein
Antibody Type Recombinant mAb
Reactivity Ms
Purification Protein A
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.

Kit


Precision Intra-assay: 5.2%;
Inter-assay: 8.8%
Sample type Cell culture supernatant, Serum, Plasma
Assay type Sandwich (quantitative)
Sensitivity 0.046ng/ml
Range 2.05 ng/mL –500 ng/mL
Recovery Cell culture supernatant: 83%
Serum: 92%
Plasma:112%
Assay time 45 minutes
Species reactivity Mouse

Background

Mouse IgG is the most abundant antibody isotype in humoral immunity, composed of two γ-heavy chains (γ-chains) and two light chains (κ or λ) linked by disulfide bonds to form a canonical Y-shaped monomeric structure. The γ-heavy chain contains three constant domains (CH1–CH3), with the Fc region (CH2–CH3) mediating binding to Fcγ receptors (FcγRI/II/III) and complement protein C1q. Unlike IgE, IgG lacks the Cε1 domain but features a hinge region that confers molecular flexibility, enhancing antigen-binding diversity. Glycosylation modifications (e.g., N-linked oligosaccharides in the Fc region) regulate IgG effector functions, such as antibody-dependent cellular cytotoxicity (ADCC). Applications of the Mouse IgG ELISA Kit: This kit is designed for quantitative detection of total IgG in mouse serum, plasma, or cell culture supernatants. Advantages of this OneStep ELISA Kit Design: Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time. High Efficiency: Achieves results within 45 minutes, ideal for high-throughput studies or urgent diagnostics. Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation. Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable IgG quantification in time-sensitive or resource-limited settings.

Picture

ELISA

Standard curve
Example of Mouse IgG standard curve in Assay Diluent #1.

Linearity
The concentrations of Mouse IgA were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Mouse Serum was 4x10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3.14 mg/mL in Mouse Serum.

Linearity
#2 sample is undiluted samples are as follows:  Mouse Plasma was 1x10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1.30 mg/mL in Mouse Plasma.

Specificity
Test the different serum specificity of IgG. All smples were 1x10^3fold dilution.

Protocol Diagram

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