Murine Gaster Tissue Digestion Solution
Murine Gaster Tissue Digestion Solution
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| Usage | 1. Before digesting the tissue sample, use ophthalmic scissors or a scalpel to cut the tissue into a volume of approximately 1-3mm.3Tissue blocks. 2. Add an appropriate volume of tissue digestive fluid (the volume of digestive fluid should be 5-10 times the volume of the original tissue) according to the size of the original tissue block; Tissue digestion is carried out in a 4 DEG C constant temperature incubator or a constant temperature shaker, and the digestion time is 15-20 minutes. Note: The digestion process must be carefully monitored, as excessive digestion may affect cell viability and significantly reduce the success rate of organoid construction. During the digestion process, the digestion suspension can be microscopically examined. Digestion can be considered complete after a large number of individual cells or cell clusters smaller than 70 μm are observed under the microscope. 3. Add 3 times primary tissue culture buffer to the tissue suspension after confirming the completion of digestion to terminate the digestion. 4. The digestion suspension obtained in the above step can be directly used for cell separation operations such as centrifugation or screen filtration. Before using the separated cells, the sample must be centrifuged and washed more than twice using organoid buffer before use (recommended centrifugation speed 200-300g for 3 minutes). |
| Synonym | Murine Gaster Tissue Digestion Solution |
| Description | The digestive fluid of primary tissue of normal mouse stomach can quickly and efficiently dissociate tissue samples into cell suspensions or cell masses for the construction of organoids. The tissue digestion solution is suitable for digestion and dissociation of rat normal gastrointestinal tissue specimens in vitro primary culture. |
| Storage Temp. | Store at -20℃ for 12 months. |