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Mouse EPO Protein, His tag

Mouse EPO Protein, His tag

Catalog Number: S0A4077 Brand: Starter
Price:
Regular price $105 USD
Regular price Sale price $105 USD
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Product Details

Product Specification


Species Mouse
Synonyms Erythropoietin
Accession P07321
Amino Acid Sequence

Protein sequence (P07321, Ala27-Arg192, with C-His tag) APPRLICDSRVLERYILEAKEAENVTMGCAEGPRLSENITVPDTKVNFYAWKRMEVEEQAIEVWQGLSLLSEAILQAQALLANSSQPPETLQLHIDKAISGLRSLTSLLRVLGAQKELMSPPDTTPPAPLRTLTVDTFCKLFRVYANFLRGKLKLYTGEVCRRGDR

Expression System HEK293
Molecular Weight Predicted MW: 20.3 kDa Observed MW: 30-40 kDa
Purity >95% by SDS-PAGE
Endotoxin <1EU/μg
Conjugation Unconjugated
Tag with C-His tag
Physical Appearance Lyophilized powder
Storage Buffer

Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4 with 3% trehalose.

Reconstitution Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
Stability & Storage

12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.

Background

Erythropoietin (EPO) is a glycoprotein hormone primarily produced by the kidneys in response to hypoxia. It stimulates red blood cell production (erythropoiesis) in the bone marrow by binding to the EPO receptor on progenitor cells. Clinically, recombinant human EPO (rhEPO) treats anemia associated with chronic kidney disease and chemotherapy. Beyond hematopoiesis, EPO exhibits tissue-protective effects in the brain and heart.

Picture

Bioactivity

Immobilized Mouse EPO Protein, His Tag at 2 μg/mL (100 μL/well) can bind Recombinant Human Erythropoietin R/EPO-R Protein, C-hFc&His with EC50 of 78-94 ng/ml.

SDS-PAGE

2μg(R: reducing conditions)

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