Mouse cutaneous cells were stained using the Mouse DC Panel Kit consisting of anti-mouse CD11c APC, anti-mouse Mouse I-A/I-E FITC, and anti-mouse CD11b PerCP-Cy5.5 conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 583 (S0B88803) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD11b PerCP-Cy5.5-A vs SSC-A dot plot and apply the CD45+ gate.
4. Create a CD11c APC-A vs SSC-A dot plot and apply the CD11b+ gate. Create gates to encompass CD11b-CD11c+I-A/I-E+ cells (cDC1), CD11b+CD11c+I-A/I-E+ cells (cDC2).
Mouse DC Panel Kit
Mouse DC Panel Kit
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$183 USD
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$183 USD
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Product Details
Product Details
Product Specification
| Application | FCM |
| Reactivity | Ms |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
This three‑color panel is designed for identifying conventional dendritic cell subsets, namely cDC1 and cDC2 in mouse tissue cells. CD11c and I‑A/I‑E (MHC class II) were included to identify dendritic cells. CD11b was included to distinguish conventional type 1 dendritic cells(cDC1) from conventional type 2 dendritic cells(cDC2) within the dendritic cells. The panel is compatible with most two‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
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