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Mouse CXCL10/IP-10/CRG-2 ELISA Kit

Mouse CXCL10/IP-10/CRG-2 ELISA Kit

Catalog Number: abs520013 Application: ELISA Reactivity: Mouse Conjugation:
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$450 USD
$450 USD
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Product Details

Product Specification

Theory Double antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse CXCL10/IP-10/CRG-2 antibodies were precoated on high-affinity microplate. After incubation, CXCL10/IP-10/CRG-2 present in the sample combined with the solid-phase antibody and the detection antibody to form an immune complex. After washing to remove the unbound material, the horseradish peroxidase labeled Streptavidin-HRP was added. After washing, the chromogenic substrate was added and the color was developed in the dark. Join terminated liquid termination reaction, in the 450 - nm wavelength (reference calibration wavelength of 540 nm or 570 nm) determination of absorbance values.
Composition
Component Size Once opened, diluted or heavy soluble reagent expiration date
Mouse CXCL10/IP-10/CRG-2 Microplate 1 Unused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants
Mouse CXCL10/IP-10/CRG-2 standard substance 2 Dissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days
Mouse CXCL10/IP-10/CRG-2 detect antibody 1 Enrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days
40×SA-HRP 1 40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save
10 x condense dilute with buffer 1 After opening, can be in 2-8 ° C stored for 30 days
Color-substrate solution 1
Stop buffer 1
20 x inspissation washing buffer 1
Sealing film 3 Room temperature storage, to avoid contamination, do not repeat use
Background Chemokine (C - X - C motif) ligand 10 (CXCL10) belongs to CXC chemokines family a small cytokines, also known as 10 - kda interferon gamma induced proteins (beta IP10 or IP - 10). In response to IFN-α, CXCL10 is secreted by several cell types. These cell types include monocytes, endothelial cells, and fibroblasts. CXCL10 is thought to have a variety of effects, such as chemoattractant to monocytes and T cells, promotion of T cell adhesion to endothelial cells, anti-tumor activity and inhibitory effects on bone marrow colony formation and angiogenesis. IP-10 was originally identified as an IFN-γ-inducible gene in monocytes, fibroblasts and endothelial cells. CRG-2, the mouse homolog of human IP-10, shares approximately 67% amino acid sequence identity with human IP-10.
General Notes 1. Please use the kit within the validity period.
2. Components of different kits and different batch kits should not be mixed.
3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. "If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used."
4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc.
5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it.
6. For scientific research only, not for in vitro diagnosis.
Storage Temp. Unopened kit, 2-8° C Storage
Test Range 62.5pg/mL-4000pg/mL

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