Standard Curve
Example of Mouse CCL22 standard curve in Assay Diluent HB2. Plotted are the background-subtracted data.
This standard curve is provided for demonstration only.
Mouse CCL22/MDC Serum & Plasma OneStep ELISA Kit
Mouse CCL22/MDC Serum & Plasma OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | Mouse CCL22 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 3.5%； Inter-assay: 4.2% |
| Sample type | Serum; EDTA Plasma,Heparin Plasma, Citrate Plasma; |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 6.45 pg/mL |
| Range | 3.9 pg/mL – 250 pg/mL |
| Recovery | Serum: 98% EDTA Plasma: 108% Heparin Plasma:103% Citrate plasma: 106% |
| Assay time | 60 minutes |
| Species reactivity | Ms |
Background
Mouse CCL22/MDC (Macrophage-Derived Chemokine), also known as ABCD-1 or STCP-1, is a CC chemokine cloned from activated mouse B cells. Its cDNA encodes a 92-amino acid precursor protein that is cleaved to yield a 68-amino acid mature, non-glycosylated polypeptide with a molecular mass of approximately 7.8 kDa. Mouse CCL22 shares 64% amino acid identity and 83% similarity with human MDC. It is expressed at high levels in dendritic cells and activated B lymphocytes, with lower expression in lung, spleen, lymph nodes, and thymocytes. CCL22 signals through the CCR4 receptor and functions as a chemoattractant for monocytes, dendritic cells, natural killer cells, and chronically activated T lymphocytes. It is induced by LPS, IL-4, and IL-13, and in T cells by TCR stimulation. Notably, CCL22 recruits regulatory T cells into tumor tissues and is expressed in many human tumors, making it a significant target in tumor immunology and inflammatory disease research.
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ELISA
Spike Recovery
The recovery of Mouse CCL22 was evaluated in Mouse serum or plasma spiked with concentrations spanning the entire assay range.
Dilution linearity
The concentrations of Mouse CCL22 were measured and interpolated from the target standard curves and corrected for sample dilution.
The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as the neat biological sample (50%). The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 374.1 pg/mL in mouse serum.
The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as the neat biological sample (50%). The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 326.1 pg/mL in mouse EDTA plasma.
The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as the neat biological sample (25%). The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 341.7 pg/mL in mouse heparin plasma.
The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as the neat biological sample (25%). The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 120.1 pg/mL in mouse citrate plasma.
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays).
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.
Determination of Minimum Detectable Dose (MDD)
The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
MDD = 2 × STDEV + AVERAGE
HOOK Effect Threshold
The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.
Protocol Diagram
