Mouse CCL22/MDC Culture Media OneStep ELISA Kit

Catalog Number: S0C3234 Reactivity: Ms Conjugation: Brand: Starter

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$120 USD

Size: 1x24T 1x48T 1x96T 5x96T 10x96T

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Product Details

Product Specification

Antigen	Mouse CCL22
Immunogen	Recombinant Protein
Antibody Type	Recombinant mAb
Reactivity	Ms
Purification	Protein A
Stability & Storage	12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

Kit

Precision	Intra-assay: 3.5%； Inter-assay: 4.2%
Sample type	Cell Culture Supernatant;
Assay type	Sandwich (quantitative)
Sensitivity	0.43 pg/mL
Range	3.9 pg/mL – 250 pg/mL
Recovery	Cell Culture Supernatant: 102%
Assay time	60 minutes
Species reactivity	Ms

Background

Mouse CCL22/MDC (Macrophage-Derived Chemokine), also known as ABCD-1 or STCP-1, is a CC chemokine cloned from activated mouse B cells. Its cDNA encodes a 92-amino acid precursor protein that is cleaved to yield a 68-amino acid mature, non-glycosylated polypeptide with a molecular mass of approximately 7.8 kDa. Mouse CCL22 shares 64% amino acid identity and 83% similarity with human MDC. It is expressed at high levels in dendritic cells and activated B lymphocytes, with lower expression in lung, spleen, lymph nodes, and thymocytes. CCL22 signals through the CCR4 receptor and functions as a chemoattractant for monocytes, dendritic cells, natural killer cells, and chronically activated T lymphocytes. It is induced by LPS, IL-4, and IL-13, and in T cells by TCR stimulation. Notably, CCL22 recruits regulatory T cells into tumor tissues and is expressed in many human tumors, making it a significant target in tumor immunology and inflammatory disease research.

Picture

ELISA

Standard Curve
Example of Mouse CCL22 standard curve in Assay Diluent B1. Plotted are the background-subtracted data.
This standard curve is provided for demonstration only.

Spike Recovery
The recovery of Mouse CCL22 was evaluated in medium spiked with concentrations spanning the entire assay range.

Dilution linearity
The concentrations of Mouse CCL22 were measured and interpolated from the target standard curves and corrected for sample dilution.
The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as the neat biological sample (20%). The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 1011.7 pg/mL in stimulated mouse spleen.

The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as the neat biological sample (100%). The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 112.7 pg/mL in unstimulated mouse spleen

Spike-and-dilution Linearity
To evaluate assay linearity, samples were spiked with high levels of Mouse CCL22 in matrices and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays).
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.

Determination of Minimum Detectable Dose (MDD)
The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
MDD = 2 × STDEV + AVERAGE

HOOK Effect Threshold
The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.