Mouse C3 ELISA Kit
Mouse C3 ELISA Kit
Product Details
Product Details
Product Specification
| protein | C3 | |||||||||||||||||||||||||||||||||
| Theory | This kit uses double antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, HRP enzyme conjugate were sequentially added to microwells pre-coated with mouse complement protein 3 (C3) capture antibody, incubated and washed in the middle, and colored with substrate TMB, which was converted to blue under the catalysis of peroxidase (HRP) and final yellow under the action of acid. There was a positive correlation between the depth of color and the mouse complement protein 3 (C3) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450nm, and the sample concentration was calculated. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | ASP; CPAMD1; Complement Protein C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Acylation stimulating protein | |||||||||||||||||||||||||||||||||
| Description | Complement protein 3, often simply referred to as C3, is a protein of the immune system that is encoded by a gene called C3. It plays a central role in the activation of the complement system. One form of C3 convertase, also known as C4b2a, is formed from isomers of the activated forms of C4 and C2. C4b2a catalyzes the cleavage of the C3 protein into C3a and C3b, produced upon activation by the classical pathway as well as the lectin pathway. C3a is an acytoxin and a precursor to some cytokines such as ASP, while C3b is a progrowth agent. Factor I can cleave C3b into C3c and C3d, the latter of which plays a role in enhancing B cell responses. In the alternative complement pathway, C3 is cleaved by C3bBb, another form of C3 convertase consisting of activated C3 (C3b) and factor B (Bb). Once C3 is activated to C3b, it exposes a reactive thioester, enabling the peptide to be covalently attached to any surface capable of providing a nucleophile, such as a primary amine or hydroxyl group. The activated C3 can then interact with factor B. Factor B is then activated by factor D to form Bb. The resulting complex, C3bBb, is known as an alternative pathway (AP) C3 convertase. |
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| Composition |
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| General Notes | 1. Carry out incubation in strict accordance with the specified time and temperature to ensure accurate results. All reagents must reach room temperature 20-25 °C prior to use. Refrigerate reagents immediately after use. 2. Incorrect plate washing may lead to inaccurate results. Make sure to drain the liquid from the wells as much as possible before adding the substrate. Do not allow the micropores to dry for too long throughout the process. 3. Clean the residual liquid and fingerprints at the bottom of the plate, otherwise it will affect the OD value. 4. The substrate color development solution should be colorless, and the substrate solution that has turned blue cannot be used. 5. Avoid cross-contamination of reagents and samples to avoid wrong results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Any reaction reagent cannot come into contact with the bleaching solvent or the strong gas emitted by the bleaching solvent. Any bleaching component will destroy the biological activity of the reagents in the kit. 8. Expired products cannot be used, and components with different item numbers and batch numbers cannot be mixed. 9. Recombinant proteins from sources other than the kit may not match the antibodies in this kit and are not recognized. 10. If the disease may be spread, all samples should be managed well, and the samples and testing devices should be handled according to the prescribed procedures. |
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| Storage Temp. | Unopened kit, stored at 4 °C, shelf life 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 15.62-1000ng/mL； |
