Flow cytometric analysis of SD Rat splenocytes labelling Rat CD28 antibody at 1/200 dilution (1 μg). Goat Anti-Mouse IgG Alexa Fluor® 647 was used as the secondary antibody. Then cells were stained with CD3 - PE Antibody separately.
Mouse Anti-Rat CD28 Antibody (S-3929)
Mouse Anti-Rat CD28 Antibody (S-3929)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD28 |
| Synonyms | T-cell-specific surface glycoprotein CD28; Cd28 |
| Location | Cell membrane |
| Accession | P31042 |
| Clone Number | S-3929 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Rt |
| Positive Sample | SD Rat splenocytes |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:200 | Rt |
Background
CD28 is a critical costimulatory receptor constitutively expressed on the surface of naïve T cells that, upon binding the ligands CD80 or CD86 on antigen-presenting cells, delivers an indispensable second signal that synergizes with TCR engagement to trigger full T-cell activation, proliferation, cytokine production (especially IL-2), metabolic reprogramming and survival by recruiting PI3K, GRB2/GADS and Lck to phosphorylate downstream effectors such as Akt, PKCθ and NF-κB, and its blockade or absence leads to anergy, whereas its engineered inclusion in CAR-T cells or therapeutic agonism is exploited to enhance anti-tumor immunity.
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