Mouse Anti-Rabbit IgG (Conformation Specific) (HRP conjugate)

Mouse Anti-Rabbit IgG (Conformation Specific) specifically recognizes and binds to rabbit IgG antibodies only in their native conformational state, without recognizing the denatured and reduced rabbit IgG heavy chains (~50 kDa) and light chains (~25 kDa) that dissociate under the reducing and denaturing conditions of Western Blot. This unique property makes it exceptionally valuable in immunoprecipitation (IP) combined with Western Blot (IP-WB) experiments, because the rabbit primary antibody used for capturing the target protein in the IP step will dissociate into heavy and light chains under the subsequent Western Blot denaturing and reducing conditions, and these fragments happen to appear within the detection region of the target protein, causing severe signal interference. When this conformation-specific antibody is used as the detection antibody (secondary antibody), it only "sees" and labels those antibody molecules that still retain their intact native conformation, thereby perfectly circumventing background band interference and ensuring clear and accurate target bands. This antibody is prepared by immunizing mice with native rabbit total IgG, followed by affinity purification, and has been optimized for use at a working concentration of 1:2000, with broad application in Western Blot experiments. It is available in various conjugated forms, including HRP labeling, making it a standardized and highly effective solution to the "heavy and light chain interference" problem in IP-WB experiments.