Flow cytometric analysis of human peripheral blood leukocytes labelling Human CD15 antibody at 1/200 dilution (1 μg) / (Right panel) compared with a Mouse IgM Isotype Control / (Left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Mouse Anti-Mouse/Human CD15 Antibody (S-R651)
Mouse Anti-Mouse/Human CD15 Antibody (S-R651)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD15 |
| Synonyms | Stage-Specific Embryonic Antigen 1, X-hapten, Lewis X, 3-FAL, CD15, SSEA1, SSEA-1 |
| Clone Number | S-R651 |
| Antibody Type | Mouse mAb |
| Isotype | IgM,k |
| Application | FCM |
| Positive Sample | Human peripheral blood leukocytes |
| Predicted Reactivity | Hu, Ms |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:200 | Hu, Ms |
Background
CD15, also known as Lewis X or SSEA-1, is a carbohydrate epitope (Galβ1→4[Fucα1→3]GlcNAc) carried primarily on glycoproteins and glycolipids of myeloid cells, granulocytes, and certain lymphocyte subsets; it serves as a key adhesion molecule that mediates selectin-dependent leukocyte rolling during inflammation, acts as a marker of mature neutrophils and Hodgkin/Reed-Sternberg cells, and its differential expression is exploited in immunohistochemistry to distinguish myeloid leukemias from lymphoid malignancies and to identify stem-cell populations in normal and neoplastic tissues.
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