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Mouse Anti-Mouse MHC Class I Antibody (S-3282)

Mouse Anti-Mouse MHC Class I Antibody (S-3282)

Catalog Number: S0B8504 Application: FCM Reactivity: Mouse Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Mouse
Antigen MHC Class I
Synonyms H-2 class I histocompatibility antigen, K-B alpha chain; H-2K(B); H2-K; H2-K1
Location Membrane
Accession P01901
Clone Number S-3282
Antibody Type Mouse mAb
Isotype IgG2b,k
Application FCM
Reactivity Ms
Positive Sample C57BL/6 mouse splenocytes
Purification Protein A
Concentration 2 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS pH7.4
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 1:200 Ms

Background

MHC Class I (H-2Kb) is a 45 kDa type I transmembrane glycoprotein encoded within the mouse MHC locus on chromosome 17, functioning as the murine ortholog of human HLA-A; it folds in the endoplasmic reticulum with a 12 kDa β2-microglobulin light chain to create a peptide-binding groove that preferentially accommodates 8–10-mer peptides bearing hydrophobic C-termini, and once loaded it is transported to the cell surface where it surveys CD8⁺ cytotoxic T lymphocytes for the presence of viral, tumor or self-peptides, thereby orchestrating rapid immune elimination of aberrant cells while maintaining tolerance through engagement of inhibitory receptors such as Ly49 and KIR.

Picture

FC

Flow cytometric analysis of C57BL/6 mouse splenocytes labelling Mouse MHC Class I antibody at 1/200 dilution (1 μg) / (Right panel) compared with a Mouse IgG2b, κ Isotype Control / (Left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45R/B220 - APC Antibody separately.