Mouse Anti-Mouse CD28 Antibody (S-5510)
Mouse Anti-Mouse CD28 Antibody (S-5510)
Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD28 |
| Location | Cell membrane |
| Accession | P31041 |
| Clone Number | S-5510 |
| Antibody Type | Mouse mAb |
| Isotype Control | S0B0617 |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:200 | Ms |
Background
CD28, a 44-kDa disulfide-linked glycoprotein, is constitutively expressed on the surface of most CD4+ and CD8+ T cells. It plays a crucial role in T cell activation and signaling by interacting with its ligands CD80 (B7.1) and CD86 (B7.2) on antigen-presenting cells. CD28 enhances T cell receptor signaling, promotes cell cycle entry, and supports the survival of T cells by upregulating anti-apoptotic proteins like Bcl-2. It also preferentially promotes TH2 differentiation, aiding B cell germinal center formation and isotype switching. CD28 signaling involves multiple pathways, including PI3K, Grb2, and NFκB activation, which collectively regulate T cell proliferation, differentiation, and effector functions.
