Flow cytometric analysis of human PBMCs (human peripheral blood mononuclear cells) fixed with 4% paraformaldehyde and permeabilized with 0.1% Tween. Cells were labeled with Anti-Human Granzyme K antibody at 1/400 dilution (0.5 μg) / (right panel) compared with a Mouse IgG1, κ Isotype Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Total viable cells, as determined by Fixable Viability Dye 452 (S0B88801), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Mouse Anti-Human Granzyme K Antibody (S-2914)
Mouse Anti-Human Granzyme K Antibody (S-2914)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Granzyme K |
| Synonyms | Fragmentin-3; Granzyme-3; NK-tryptase-2 (NK-Tryp-2); TRYP2; GZMK |
| Location | Secreted |
| Accession | P49863 |
| Clone Number | S-2914 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Isotype Control | S0B0617 |
| Application | ICFCM |
| Reactivity | Hu |
| Positive Sample | Human PBMCs |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1:400 | Hu |
Background
Granzyme K is a serine protease stored in the cytotoxic granules of CD8⁺ T cells and NK cells that, upon perforin-mediated entry into target cells, cleaves the nucleosome-assembly factor SET and the DNA damage-response factor Ku70 to trigger caspase-independent nuclear condensation, mitochondrial perturbation, and rapid cell death, thereby amplifying antiviral and antitumor immunity.
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