Flow cytometric analysis of fresh human peripheral blood cells labeled with Anti-Human CD62P antibody at 1/200 dilution (1 μg) / (right panel) compared with a Mouse IgG1, κ Isotype Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD41 – APC antibody separately. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Mouse Anti-Human CD62P Antibody (S-5597)
Mouse Anti-Human CD62P Antibody (S-5597)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD62P |
| Synonyms | P-selectin; CD62 antigen-like family member P; Granule membrane protein 140 (GMP-140); Leukocyte-endothelial cell adhesion molecule 3 (LECAM3); Platelet activation dependent granule-external membrane protein (PADGEM); GMRP; GRMP; SELP |
| Location | Cell membrane |
| Accession | P16109 |
| Clone Number | S-5597 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Isotype Control | S0B0617 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Fresh human peripheral blood |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:200 | Hu |
Background
CD62P, also known as P-selectin, is a 140 kDa type I transmembrane glycoprotein that plays a crucial role in cell adhesion and immune responses. It is stored in α-granules of platelets and Weibel-Palade bodies of endothelial cells, and is rapidly relocated to the cell surface upon activation by stimuli such as thrombin or histamine. CD62P mediates the initial adhesion and rolling of leukocytes on endothelial cells during inflammation and facilitates interactions between activated platelets and neutrophils or monocytes. It binds to specific carbohydrate ligands on leukocytes in a calcium-dependent manner. Additionally, CD62P is used as a marker for platelet activation and has potential diagnostic value in various diseases.
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