Flow cytometric analysis of human peripheral blood leukocyte labelling Human CD32 antibody at 1/200 (1 μg) dilution/ (Right panel) compared with a Mouse IgG2b, κ Isotype Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD19 - Brilliant Violet 421™ antibody a separately.
Mouse Anti-Human CD32 (FcyRllA) Antibody (S-R392)
Mouse Anti-Human CD32 (FcyRllA) Antibody (S-R392)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD32 (FcyRllA) |
| Synonyms | Low affinity immunoglobulin gamma Fc region receptor II-a; IgG Fc receptor II-a; CDw32; Fc-gamma RII-a (Fc-gamma-RIIa; FcRII-a); FCG2; FCGR2A1; IGFR2; FCGR2A |
| Location | Cell membrane |
| Accession | P12318 |
| Clone Number | S-R392 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human peripheral blood leukocyte |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:200 | Hu |
Background
CD32 (FcγRIIA) is a 40-kDa type I transmembrane glycoprotein of the Ig superfamily expressed on monocytes/macrophages, granulocytes, dendritic cells, B cells, platelets, and placental endothelium; it contains two extracellular Ig-like domains and functions as a low-affinity receptor for complexed/aggregated IgG (Kd < 10⁻⁷ M) that mediates phagocytosis, respiratory burst, ADCC, and immune complex clearance, with the FcγRIIA (CD32a) isoform delivering activating signals via an intracellular ITAM motif, while the FcγRIIB (CD32b) isoform delivers inhibitory signals via an ITIM motif, and polymorphisms and alternative splicing of the FCGR2A/FCGR2B/FCGR2C genes generate multiple isoforms with distinct expression patterns and functional roles .
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