Monoamine Oxidase B/MAOB Recombinant Rabbit mAb (S-5317)

Catalog Number: S0B60186 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Monoamine Oxidase B/MAOB
Synonyms	Amine oxidase [flavin-containing] B
Location	Mitochondrion
Accession	P27338
Clone Number	S-5317
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	HepG2, MCF7, SH-SY5Y
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:500-1:1000	Hu
IHC-P	1:250	Hu

Background

Monoamine oxidase B (MAO-B) is an enzyme primarily found in the brain and platelets that catalyzes the oxidative deamination of neuroactive and vasoactive amines, particularly dopamine. By breaking down dopamine, MAO-B plays a key role in regulating its synaptic levels, making it an important pharmacological target—MAO-B inhibitors (e.g., selegiline, rasagiline) are used as adjunct therapies in Parkinson’s disease to enhance dopaminergic neurotransmission and provide neuroprotective effects.

Picture

Western Blot

WB result of Monoamine Oxidase B/MAOB Recombinant Rabbit mAb
Primary antibody: Monoamine Oxidase B/MAOB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A204 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Negative control: A204 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 60 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-MAOB antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-MAOB antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-MAOB antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-MAOB antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-MAOB antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.