Monkey TSLP Culture Media OneStep ELISA Kit

Catalog Number: S0C3232 Reactivity: Mk Conjugation: Brand: Starter

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$120 USD

Size: 1x24T 1x48T 1x96T 5x96T 10x96T

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Product Details

Product Specification

Antigen	Monkey TSLP
Immunogen	Recombinant Protein
Antibody Type	Recombinant mAb
Reactivity	Mk
Purification	Protein A
Stability & Storage	12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

Kit

Precision	Intra-assay: 4.9%； Inter-assay: 5.1%
Sample type	Cell Culture Supernatant;
Assay type	Sandwich (quantitative)
Sensitivity	23.88 pg/mL
Range	31.3 pg/mL – 2000 pg/mL
Recovery	Cell Culture Supernatant: 88%
Assay time	60 minutes
Species reactivity	Mk

Background

Monkey TSLP (Thymic Stromal Lymphopoietin) is a ~140 amino acid, 16 kDa cytokine of the IL-2 family that contains a furin cleavage site (127-RRKR-130), yielding an ~11.7 kDa N-terminal and ~5.4 kDa C-terminal fragment, and is commonly produced as a C-terminal His-tagged recombinant protein spanning Tyr29–Gln159. Because the TSLP pathway is conserved between humans and cynomolgus monkeys, monkey TSLP serves as a key translational model for human allergic disease, particularly asthma, where TSLPR blockade significantly reduced bronchoalveolar lavage eosinophils, lung resistance, IL-13 levels, and the TSLP-DC gene signature, confirming that TSLP-driven dendritic cell activation plays a central role in allergic inflammation.

Picture

ELISA

Standard Curve
Example of Monkey TSLP standard curve in Assay Diluent M1. Plotted are the background-subtracted data.
This standard curve is provided for demonstration only.

Spike Recovery
The recovery of Monkey TSLP was evaluated in medium spiked with concentrations spanning the entire assay range.

Spike-and-dilution Linearity
To evaluate assay linearity, samples were spiked with high levels of Monkey TSLP in matrices and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays).
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.

Determination of Minimum Detectable Dose (MDD)
The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
MDD = 2 × STDEV + AVERAGE

HOOK Effect Threshold
The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.