Standard curve
Example of Monkey TL1A/TNFSF15 standard curve in Assay Diluent DA4.
Monkey TL1A/TNFSF15 OneStep ELISA Kit
Monkey TL1A/TNFSF15 OneStep ELISA Kit
Price:
Regular price
$500 USD
Regular price
Sale price
$500 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Antigen | TL1A/TNFSF15 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Mk |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 2.0%； Inter-assay: 3.5% |
| Sample type | Cell culture supernatant; Serum; Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 1.757 pg/mL |
| Range | 7.81 pg/mL – 500 pg/mL |
| Recovery | Cell culture supernatant: 113% Serum: 87% Plasma: 94% |
| Assay time | 60 minutes |
| Species reactivity | Mk |
| CROSS REACTIVITY | Hu |
Background
Monkey TL1A, also known as TNFSF15, is a protein belonging to the tumor necrosis factor (TNF) superfamily. It is primarily expressed on the surface of vascular endothelial cells and certain immune cells. This protein functions as a ligand for the death domain receptor 3 (DR3) on lymphocytes. Upon binding, it activates downstream signaling pathways such as NF-κB and MAPK, which promote inflammatory responses. Its primary role is involved in modulating immune functions, including T cell activation and proliferation. Research indicates that TL1A is a key player in the pathogenesis of various chronic inflammatory and autoimmune diseases, particularly those related to vascular inflammation. Consequently, it has been identified as a potential therapeutic target, with monoclonal antibodies against TL1A currently under investigation for treating conditions like inflammatory bowel disease and rheumatoid arthritis.
Picture
Picture
ELISA
Linearity
The concentrations of Monkey TL1A/TNFSF15 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: Monkey serum was 2-fold dilution (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 83.56 pg/mL in monkey serum.
Sample is undiluted samples are as follows: Monkey plasma was 2-fold dilution (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 71.88 pg/mL in monkey plasma.
To assess the linearity of the assay, sample were spiked with high concentrations of Monkey TL1A/TNFSF15 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
To assess the linearity of the assay, two samples were spiked with high concentrations of Monkey TL1A/TNFSF15 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Protocol Diagram
