Standard curve
Example of Monkey IL-8 standard curve in Assay Diluent E1.
Monkey IL-8 Onestep ELISA Kit
Monkey IL-8 Onestep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | lL-8/CXCL8 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Mk |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 6.5%； Inter-assay: 6.7% |
| Sample type | Cell culture supernatant; Serum; Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.444 pg/mL |
| Range | 3.13 pg/mL – 200 pg/mL |
| Recovery | Cell culture supernatant: 90% Serum: 111% Plasma: 109% |
| Assay time | 60 minutes |
| Species reactivity | Mk |
Background
Monkey Interleukin-8 (IL-8) is a pivotal CXC chemokine in non-human primates, functionally conserved and serving as the primary chemoattractant and activator for neutrophils during innate immune responses. It is rapidly released in response to inflammatory stimuli such as infection or injury, directing neutrophil migration, adhesion, and antimicrobial activity to the affected site. This protein is also involved in angiogenesis, influencing both wound healing and pathological processes. Due to its high biological similarity to human IL-8, it is an indispensable translational biomarker in biomedical research, extensively utilized to assess inflammation, evaluate drug safety, study infectious diseases, and develop vaccines in preclinical primate models.
Picture
Picture
ELISA
Linearity
The concentrations of Monkey IL-8 were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Monkey PBMC cells stimulated with 10ug/mL PHA for 5days (0.5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 46.70 ng/mL in stimulated monkey PBMC supernatant.
#2 sample is undiluted samples are as follows: Monkey PBMC cells culture for 5days (2%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 6.87 ng/mL in unstimulated monkey PBMC supernatant.
#3 sample is undiluted samples are as follows: Monkey PBMC cells stimulated with 2 ug/mL poly I:C for 24 hours (20%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 807.09 pg/mL in stimulated monkey PBMC supernatant.
#4 sample is undiluted samples are as follows: Monkey PBMC cells culture for 24 hours (40%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 306.61 pg/mL in unstimulated monkey PBMC supernatant.
#5 sample is undiluted samples are as follows: Female monkey serum was 50fold dilution (2%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 7.13 ng/mL in female monkey serum.
#6 sample is undiluted samples are as follows: Male monkey serum was 50fold dilution (2%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 6.64 ng/mL in male monkey serum.
#7 sample is undiluted samples are as follows: Monkey plasma (EDTA) was 10fold dilution (10%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 799.05 pg/mL in monkey plasma (EDTA).
Specificity
Test the specificity of different serum.
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Protocol Diagram
