Standard curve
Example of Monkey IL-5 standard curve in Assay Diluent DA4.
Monkey IL-5 OneStep ELISA Kit
Monkey IL-5 OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IL-5 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Mk |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 1.9%； Inter-assay:3.4% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 2.631 pg/mL |
| Range | 9.38 pg/mL – 600 pg/mL |
| Recovery | Cell culture supernatant: 100% Serum: 91% Plasma: 91% |
| Assay time | 60 minutes |
| Species reactivity | Hu |
Background
Interleukin (IL)-5 is a cytokine dimer derived from both hematopoietic and non-hematopoietic cell lines that primarily contribute to the proliferation, differentiation, and survival of eosinophils. While other cytokines have been implicated in the maturation, survival, and function of eosinophils, as one of its primary roles includes stimulating production of these cells from the bone marrow. Given eosinophilic prominence in a variety of disease states, considerable research has focused on the development of therapeutic drugs targeting IL-5-dependent signaling.
Picture
Picture
ELISA
Linearity
The concentrations of Monkey IL-5 were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Monkey PBMC cells stimulated with 10ug/ml PHA for 5days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 24.10 pg/mL in stimulated Monkey PBMC supernatant.
#2 sample is undiluted samples are as follows: Monkey PBMC cells culture for 5days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 4.91 pg/mL in unstimulated Monkey PBMC supernatant.
Specificity
Test the specificity of Mouse IL-5 Standard.
Protocol Diagram
