Standard curve
Example of Monkey IgG standard curve in Assay Diluent #1.
Monkey IgG competitive OneStep ELISA Kit
Monkey IgG competitive OneStep ELISA Kit
Price:
Regular price
$500 USD
Regular price
Sale price
$500 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Antigen | IgG |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 4.8%； Inter-assay: 6.7% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Competitive |
| Sensitivity | ＜2.05 ug/mL |
| Range | 2.05 ug/mL – 500 ug/mL |
| Recovery | Cell culture supernatant: 105% Serum: 97% Plasma: 103% |
| Assay time | 60 minutes |
| Species reactivity | Monkey |
Background
Monkey IgG, like other mammalian immunoglobulins G (IgG), is a Y-shaped glycoprotein composed of two identical heavy chains (γ-chains) and two identical light chains (κ or λ). Each chain contains constant (C) and variable (V) regions. The variable regions form the antigen-binding fragment (Fab), responsible for recognizing specific antigens, while the constant region (Fc) mediates immune effector functions such as complement activation and binding to Fc receptors on immune cells. Monkey IgG shares structural similarities with human IgG, particularly in conserved domains like the Fc region, which is critical for cross-species applications.
Picture
Picture
ELISA
Linearity
The concentrations of Monkey IgG were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: male Monkey serum (2.5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 16.27 mg/mL in male monkey serum. #2 sample is undiluted samples are as follows: female monkey serum (2.5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 11.68 mg/mL in female monkey serum.
Protocol Diagram
