2μg Modified Trypsin, Mass Spectrometry Grade
R: reducing condition
Modified Trypsin, Mass Spectrometry Grade
Modified Trypsin, Mass Spectrometry Grade
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$285 USD
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Product Details
Product Details
Product Specification
| Synonyms | Trypsin(pig); porcine pancreas; Trypsin，Mass Spectrometry Grade |
| Expression System | E.coli |
| Molecular Weight | 24±2 kDa (Reducing) |
| Purity | ＞95% by RP- HPLC |
| Tag | No Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | 2 mM HCl, 2 mM Ca2+ |
| Reconstitution | Modified Trypsin, Mass Spectrometry Grade should be reconstituted by the addition of 20~200 μL of 2 mM HCl or 50mM acetic acid. |
| Stability & Storage | Store at -25 ~ -15℃ for 2 years |
| Reference | Olsen, J. V. Trypsin Cleaves Exclusively C-terminal to Arginine and Lysine Residues[J]. Molecular & Cellular Proteomics, 2004, 3(6):608-614. |
Background
Modified Trypsin, Mass Spectrometry Grade is produced through a multi-step process: the wild-type trypsin sequence is engineered to minimize autolysis, the modified enzyme is then expressed recombinantly to eliminate contaminating proteases, and finally subjected to reductive methylation to further enhance stability. The resulting product exhibits high purity and exceptional stability, making it ideal for a broad range of laboratory proteomics applications, including protein/peptide sequencing and identification, as well as HPLC peptide mapping.
Protocol
1 Dissolve Modified Trypsin by adding 20 to 200 μL of 2 mM HCL or 50 mM acetic acid, and prepare 2*Reaction buffer (100 mM Tris-HCl, 10 mM CaCl2, pH 8.0 @ 25°C)
2 Set-up a typical reaction as follows
1）Add the following components in sequence
Components |
Volume |
Substrate protein |
5 μL (about 2 ug) |
ddH2O |
4.5 μL |
2*Reaction buffer |
10 μL |
100 ng/μL UA070137 |
0.5 μL |
2）Incubate at 37°C for 2~4 h or overnight
Guidelines
1. Substrate must be in phosphate-free buffer to prevent calcium precipitation with both reconstituted enzyme and enzyme buffer.
2. Storage buffer:2 mM HCl or 50mM acetic acid
Unit Definition
In a 3.2 mL reaction system using BAEE as substrate, the UV absorption at 253 nm increased by 0.003 per min using cuvette with 1 cm optical path, which was defined as 1 USP trypsin activity unit
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Bioactivity
SDS-PAGE
RP-HPLC
