MMP8 Recombinant Rabbit mAb (S-6026)

Catalog Number: S0B60797 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	MMP8
Location	Secreted
Accession	P22894
Clone Number	S-6026
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	Mouse spleen, mouse lung, rat lung
Purification	Protein A
Concentration	0.25 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Ms, Rt
IHC-P	1:3000-1:6000	Hu, Ms, Rt

Background

MMP8 (matrix metalloproteinase-8, also known as neutrophil collagenase) is a zinc-dependent endopeptidase of 467 amino acids encoded by the MMP8 gene in the q22.2 region of human chromosome 11, belonging to the matrix metalloproteinase family, with its classical function being the degradation of type I, II, and III fibrillar collagens. Its catalytic domain is spherical, and the bottom of the active-site cleft contains a catalytic zinc ion that is pentacoordinated by three histidines and the two hydroxamate oxygen atoms of an inhibitor; in addition, it contains a structural zinc ion and two calcium ions that stabilize the structure. MMP8 has traditionally been regarded as a neutrophil-specific enzyme, stored in granulocyte granules and rapidly released upon stimulation, but subsequent studies have shown that endothelial cells, smooth muscle cells, and macrophages can also express it upon stimulation by inflammatory factors. At the pathophysiological level, the effects of MMP8 are markedly context-dependent: in atherosclerosis, macrophage-derived active MMP8 within plaques is closely associated with plaque instability and rupture; whereas in various cancers, a high density of tumor-associated MMP8⁺ cells is instead associated with good prognosis and local immune activity in colorectal cancer, although elevated serum MMP8 reflects systemic inflammation and poor survival. In addition, in a mouse model of chronic stress, circulating monocyte-derived MMP8 can infiltrate the nucleus accumbens and regulate extracellular space ultrastructure and social avoidance behavior.

Picture

Western Blot

WB result of MMP8 Recombinant Rabbit mAb
Primary antibody: MMP8 Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: mouse spleen lysate 20 μg
Lane 2: mouse lung lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 55 kDa

WB result of MMP8 Recombinant Rabbit mAb
Primary antibody: MMP8 Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: rat lung lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 55 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human liver. Anti-MMP8 antibody was used at 1/6000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human spleen. Anti-MMP8 antibody was used at 1/6000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-MMP8 antibody was used at 1/6000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-MMP8 antibody was used at 1/6000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse spleen. Anti-MMP8 antibody was used at 1/6000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat lung. Anti-MMP8 antibody was used at 1/6000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat spleen. Anti-MMP8 antibody was used at 1/6000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.