MMP-8 Recombinant Rabbit mAb (S-5674)

Catalog Number: S0B60405 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	MMP-8
Synonyms	Neutrophil collagenase; Matrix metalloproteinase-8 (MMP-8); PMNL collagenase (PMNL-CL); CLG1; MMP8
Location	Secreted
Accession	P22894
Clone Number	S-5674
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	mouse spleen, mouse lung, rat lung
Purification	Protein A
Concentration	2 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Ms, Rt
IHC-P	1:2000	Hu, Ms, Rt

Background

MMP-8, also known as neutrophil collagenase, is a zinc-dependent metalloproteinase encoded by the MMP8 gene. It is primarily expressed in neutrophils and is one of the key enzymes that initiates the degradation of fibrillar collagens, particularly type I collagen. In terms of physiological function, MMP-8 is not only involved in neural crest cell migration and tissue remodeling during embryonic development, but also plays a significant role in pathological processes such as atherosclerosis due to its potent collagenolytic activity—it degrades collagen in the fibrous cap of plaques, which is associated with plaque instability and increased risk of cardiovascular disease. In cancer research, the role of MMP-8 is complex, exhibiting both tumor-suppressive and tumor-promoting properties depending on the tumor type and microenvironment. As one of the most potent interstitial collagenases in degrading collagen, MMP-8 activity is finely regulated in vivo, making it a key participant in various inflammatory diseases and matrix remodeling-related disorders, as well as a potential therapeutic target.

Picture

Western Blot

WB result of MMP-8 Recombinant Rabbit mAb
Primary antibody: MMP-8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse lung lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 60 kDa

WB result of MMP-8 Recombinant Rabbit mAb
Primary antibody: MMP-8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat lung lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 60 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human spleen. Anti-MMP-8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-MMP-8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-MMP-8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse spleen. Anti-MMP-8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat spleen. Anti-MMP-8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.