The MLK3 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the MLK3 protein , ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU with BMG.
MLK3 Protein
MLK3 Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | MAP3K11, PTK1, SPRK |
| Accession | Q16584-1 |
| Amino Acid Sequence |
Y99-G398，N-terminal GST tag
YVSRGGGPPPCEVASFQELRLEEVIGIGGFGKVYRGSWRGELVAVKAARQDPDEDISVTAESVRQEARLFAMLAHPNIIALKAVCLEEPNLCLVMEYAAGGPLSRALAGRRVPPHVLVNWAVQIARGMHYLHCEALVPVIHRDLKSNNILLLQPIESDDMEHKTLKITDFGLAREWHKTTQMSAAGTYAWMAPEVIKASTFSKGSDVWSFGVLLWELLTGEVPYRGIDCLAVAYGVAVNKLTLPIPSTCPEPFAQLMADCWAQDPHRRPDFASILQQLEALEAQVLREMPRDSFHSMQEG |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 59.8 kDa |
| Purity | >80% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | GST Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 20 mM GSH, 1 mM DTT, 10% glycerol, 0.05% Brij-35, pH7.5 |
| Stability & Storage |
Stable for
12 months upon stored at -80℃ from the date of receipt. And avoid repeated
freeze-thaws cycles.
|
Picture
Picture
Bioactivity
SDS-PAGE
