WB result of Mitofusin 2 Recombinant Rabbit mAb
Primary antibody: Mitofusin 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: Raji whole cell lysate 20 µg
Lane 6: SK-OV-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 80 kDa
Mitofusin 2 Recombinant Rabbit mAb (S-1783-49)
Mitofusin 2 Recombinant Rabbit mAb (S-1783-49)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Mitofusin 2 |
| Synonyms | Mitofusin-2; Transmembrane GTPase MFN2; CPRP1; KIAA0214; MFN2 |
| Immunogen | Recombinant Protein |
| Location | Mitochondrion |
| Accession | O95140 |
| Clone Number | S-1783-49 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HEK-293, HepG2, Jurkat, Raji, SK-OV-3, NIH/3T3, mouse brain , mouse kidney, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms |
| ICC | 1:500 | Hu |
Background
Mitofusin 2 (MFN2) is a dynamin-like GTPase protein anchored to the outer mitochondrial membrane, playing a crucial role in mitochondrial fusion, fission, and tethering to the endoplasmic reticulum (ER). It helps maintain mitochondrial morphology and function, and regulates intracellular calcium homeostasis and lipid metabolism. MFN2 is also involved in mitophagy by interacting with key proteins like PINK1 and Parkin, which mediate the selective elimination of damaged mitochondria. Mutations in the MFN2 gene are associated with Charcot–Marie–Tooth disease type 2A, a neurological disorder characterized by axonal degeneration and muscle atrophy. Additionally, MFN2 has been implicated in various cellular processes, including the regulation of the NLRP3 inflammasome and the maintenance of cell homeostasis. Its dual localization and diverse functions make it a critical regulator in both mitochondrial dynamics and interorganellar communication.
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Western Blot
WB result of Mitofusin 2 Recombinant Rabbit mAb
Primary antibody: Mitofusin 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Lane 3: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 80 kDa
WB result of Mitofusin 2 Recombinant Rabbit mAb
Primary antibody: Mitofusin 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 80 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-Mitofusin 2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Mitofusin 2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-Mitofusin 2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Mitofusin 2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-Mitofusin 2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-Mitofusin 2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti-Mitofusin 2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
