mIHC Dye (TSA540)
mIHC Dye (TSA540)
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$498 USD
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Product Details
Product Details
Description
mIHC Dye (TSA540) is a high-performance fluorescent dye designed for multiplex immunohistochemistry (mIHC) based on tyramide signal amplification (TSA) technology. It enables robust, covalent fluorescent labeling on formalin-fixed paraffin-embedded (FFPE) tissues, frozen sections, and tissue microarray (TMA) slides, supporting multi-target, in situ biomarker detection in a single tissue section.
This reagent provides strong, stable fluorescence with Ex/Em: 520 nm / 540 nm, compatible with standard fluorescence microscopes and multispectral imaging systems. It delivers greatly amplified signals compared to conventional immunofluorescence, ideal for low-abundance antigens and spatial profiling of the tissue microenvironment.
Key Features
TSA-based signal amplification: Ensures high sensitivity and high signal-to-noise ratio, suitable for detecting low-abundance antigens.
Covalent binding: Binds to tyrosine residues covalently, maintaining excellent stability during antibody stripping and repeated staining cycles.
Broad Compatibility: Applicable to FFPE tissues, frozen sections, and TMA slides, meeting diverse experimental needs.
Spectral Characteristics: 540 nm spectral channel, matching AF514, Opal 540, and similar orange-yellow fluorophores.
Easy to Use: Ready-to-use formulation with included signal amplification reaction buffer, no complex pre-processing required.
Application Scope: For research use only; not for in vitro clinical diagnosis or human trials.
Principle
TSA540 adopts horseradish peroxidase (HRP)-conjugated secondary antibodies to activate fluorescently labeled tyramide substrates. The activated tyramide rapidly forms covalent bonds with tyrosine residues near the antigen-antibody complex, forming stable fluorescent deposits. After that, the antibodies can be stripped by heat repair without removing the dye signal, allowing sequential labeling of multiple targets on the same tissue section to achieve multiplex staining.
Storage & Stability
Storage Condition: Store protected from light at 2–8 °C; avoid direct exposure to strong light.
Shelf Life: Stable for 12 months from the date of receipt when stored according to the required conditions.
Composition
TSA Monochromatic Fluorescent Dye 540, Signal Amplification Reaction Buffer
Application
Mainly used for scientific research of immunohistochemical staining of tissues, paraffin sections, and TMA chips; not for in vitro clinical diagnosis or human trials.
