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MICB His Tag Protein, Human

MICB His Tag Protein, Human

Catalog Number: UA010590 Reactivity: Human Conjugation: Unconjugated Brand: UA BIOSCIENCE
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Product Details

Product Specification


Species Human
Synonyms PERB11.2
Accession Q29980-1
Amino Acid Sequence

Ala23-Asp309, with C-terminal 8*His AEPHSLRYNLMVLSQDGSVQSGFLAEGHLDGQPFLRYDRQKRRAKPQGQWAENVLGAKTWDTETEDLTENGQDLRRTLTHIKDQKGGLHSLQEIRVCEIHEDSSTRGSRHFYYDGELFLSQNLETQESTVPQSSRAQTLAMNVTNFWKEDAMKTKTHYRAMQADCLQKLQRYLKSGVAIRRTVPPMVNVTCSEVSEGNITVTCRASSFYPRNITLTWRQDGVSLSHNTQQWGDVLPDGNGTYQTWVATRIRQGEEQRFTCYMEHSGNHGTHPVPSGKALVLQSQRTDGGGSHHHHHHHH
Expression System HEK293
Molecular Weight 42-55kDa
Purity ＞95% by SDS-PAGE
Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized Powder
Storage Buffer PBS, pH7.4
Reconstitution Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1． Gene ID: 4277, updated on 21-Jun-2023.

Background

This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. Similar to classical MHC class I proteins, MICB contains three Ig-like domains (alpha 1, alpha 2, and alpha 3) in the extracellular domain (ECD), a transmembrane segment, and a carboxy-terminal cytoplasmic tail. MICB is minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections.

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SDS-PAGE

2μg (R: reducing conditions, N: non-reducing conditions).

ELISA

Immobilized MICB His Tag, Human (Cat. No. UA010590) at 2.0μg/mL (100μL/well) can bind NKG2D/CD314 Fc Chimera, Human (Cat. No. UA010732) with EC50 of 0.48-0.61μg/mL.

Immobilized MICB His Tag, Human (Cat. No. UA010590) at 2.0μg/mL (100μL/well) can bind NKG2D/CD314 Fc Chimera, Cynomolgus/Rhesus (Cat. No. UA010739) with EC50 of 0.14-0.18 μg/mL.

SPR

Protein A Chip captured NKG2D/CD314 Fc Chimera, Human (Cat. No. UA010732), can bind MICB His Tag, Human (Cat. No. UA010590) with an affinity constant of 47.67nM as determined in SPR assay.