2μg (R: reducing conditions, N: non-reducing conditions).
MICB His Tag Protein, Human
MICB His Tag Protein, Human
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$592.00 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | PERB11.2 |
| Accession | Q29980-1 |
| Amino Acid Sequence | Ala23-Asp309, with C-terminal 8*His AEPHSLRYNLMVLSQDGSVQSGFLAEGHLDGQPFLRYDRQKRRAKPQGQWAENVLGAKTWDTETEDLTENGQDLRRTLTHIKDQKGGLHSLQEIRVCEIHEDSSTRGSRHFYYDGELFLSQNLETQESTVPQSSRAQTLAMNVTNFWKEDAMKTKTHYRAMQADCLQKLQRYLKSGVAIRRTVPPMVNVTCSEVSEGNITVTCRASSFYPRNITLTWRQDGVSLSHNTQQWGDVLPDGNGTYQTWVATRIRQGEEQRFTCYMEHSGNHGTHPVPSGKALVLQSQRTDGGGSHHHHHHHH |
| Expression System | HEK293 |
| Molecular Weight | 42-55kDa |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1． Gene ID: 4277, updated on 21-Jun-2023. |
Background
This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. Similar to classical MHC class I proteins, MICB contains three Ig-like domains (alpha 1, alpha 2, and alpha 3) in the extracellular domain (ECD), a transmembrane segment, and a carboxy-terminal cytoplasmic tail. MICB is minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections.
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SDS-PAGE
ELISA
Immobilized MICB His Tag, Human (Cat. No. UA010590) at 2.0μg/mL (100μL/well) can bind NKG2D/CD314 Fc Chimera, Human (Cat. No. UA010732) with EC50 of 0.48-0.61μg/mL.
Immobilized MICB His Tag, Human (Cat. No. UA010590) at 2.0μg/mL (100μL/well) can bind NKG2D/CD314 Fc Chimera, Cynomolgus/Rhesus (Cat. No. UA010739) with EC50 of 0.14-0.18 μg/mL.
SPR
Protein A Chip captured NKG2D/CD314 Fc Chimera, Human (Cat. No. UA010732), can bind MICB His Tag, Human (Cat. No. UA010590) with an affinity constant of 47.67nM as determined in SPR assay.
