WB result of MICA/MICB Recombinant Rabbit mAb
Primary antibody: MICA/MICB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HCT 116 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HeLa treated with PNGase F whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Lane 6: HUVEC whole cell lysate 20 µg
Lane 7: THP-1 whole cell lysate 20 µg
Negative control: THP-1 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 35-100 kDa
MICA/MICB Recombinant Rabbit mAb (S-1589-10)
MICA/MICB Recombinant Rabbit mAb (S-1589-10)
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$100.00 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MICA/MICB |
| Synonyms | MHC class I polypeptide-related sequence A; MIC-A; PERB11.1; |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Cell membrane, Endoplasmic reticulum |
| Accession | Q29983、 Q29980 |
| Clone Number | S-1589-10 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | HCT 116, HeLa, A431, MCF7, HUVEC |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
MICA (MHC class I polypeptide-related sequence A) and MICB (MHC class I polypeptide-related sequence B) are stress-inducible proteins that belong to the MHC class I family but do not bind to β2-microglobulin or present peptides. They are encoded by genes located within the HLA class I region of chromosome 6. MICA and MICB are expressed on the cell membrane in response to cellular stress or cancer transformation. These proteins act as ligands for the NKG2D receptor, which is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells. The binding of MICA/B to NKG2D can activate these immune cells, leading to cytotoxic responses against stressed or transformed cells. MICA and MICB are highly polymorphic, with over 106 alleles reported for MICA and 25 for MICB. This polymorphism may affect their affinity for NKG2D and, consequently, the activation thresholds of NK and T cells.
Picture
Picture
Western Blot
WB result of MICA/MICB Recombinant Rabbit mAb
Primary antibody: MICA/MICB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Purified human MICB recombinant protein 10 ng
Lane 2: Purified human MICA recombinant protein 10 ng
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 37-70 kDa
