WB result of MFF Recombinant Rabbit mAb
Primary antibody: MFF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: DU 145 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Lane 5: T-47D whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 25-45 kDa
MFF Recombinant Rabbit mAb (S-2330-28)
MFF Recombinant Rabbit mAb (S-2330-28)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MFF |
| Synonyms | Mitochondrial fission factor; C2orf33 |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | Q9GZY8 |
| Clone Number | S-2330-28 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | MCF7, DU 145, A549, HeLa, T-47D, mouse brain, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
MFF (Mitochondrial Fission Factor) is an evolutionarily conserved, tail-anchored outer-mitochondrial-membrane receptor that serves as the principal platform for recruiting the cytosolic GTPase Drp1 to sites of future organelle division, thereby initiating the oligomerization of Drp1 into a helical collar that constricts and finally severs the mitochondrion in a GTP-driven reaction, while also integrating peroxisomal fission, controlling mitochondrial size, number and distribution, and acting as a crucial node for post-translational and lipid-mediated signals that tune the balance between fission and fusion in response to metabolic stress, cell-cycle cues and pathological stimuli such as those observed in cancer and cardiovascular disease.
Picture
Picture
Western Blot
WB result of MFF Recombinant Rabbit mAb
Primary antibody: MFF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 35 kDa
This blot was developed with high sensitivity substrate
WB result of MFF Recombinant Rabbit mAb
Primary antibody: MFF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 35, 48 kDa
This blot was developed with high sensitivity substrate
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-MFF antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
