METTL1 Recombinant Rabbit mAb (S-4435-6)

Catalog Number: S0B60185 Application: WB, IHC-P Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	METTL1
Synonyms	tRNA (guanine-N(7)-)-methyltransferase; Methyltransferase-like protein 1; mRNA (guanine-N(7)-)-methyltransferase; miRNA (guanine-N(7)-)-methyltransferase; tRNA (guanine(46)-N(7))-methyltransferase; tRNA(m7G46)-methyltransferase; C12orf1
Immunogen	Recombinant Protein
Location	Nucleus
Accession	Q9UBP6
Clone Number	S-4435-6
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	HeLa, 293T, HepG2, A549, Caco-2, HaCaT, A431, HCT 116, NIH/3T3, RAW264.7, PC-12, rat brain, rat testis, COS-7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Hu, Ms, Rt, Mk
IHC-P	1:2000	Hu, Ms, Rt

Background

METTL1 is a member of the S-adenosylmethionine (SAM)-dependent methyltransferase family, primarily responsible for catalyzing the generation of N7-methylguanosine (m⁷G) modifications in tRNA. This protein typically forms a stable heterodimeric complex with WDR4, in which METTL1 serves as the catalytic subunit providing methyltransferase activity, while WDR4 acts as the auxiliary subunit responsible for substrate recognition and complex stability. At the functional level, METTL1-mediated tRNA m⁷G modification is essential for maintaining tRNA structural stability and regulating translation efficiency; this modification can influence the binding efficiency of tRNA to ribosomes by affecting its spatial conformation, thereby modulating global protein synthesis rates. Additionally, METTL1 is also involved in regulating the splicing of mRNA precursors containing m⁷G cap structures and plays important roles in various biological processes including cell cycle regulation, embryonic stem cell self-renewal, and DNA damage response. In terms of disease associations, METTL1 is upregulated in multiple tumors such as hepatocellular carcinoma, colorectal cancer, and lung cancer, where it promotes the translation of oncogenic mRNAs by enhancing tRNA m⁷G modification levels, thereby driving tumor cell proliferation and metastasis, making it a promising anticancer drug target. Furthermore, mutations or aberrant expression of METTL1 have also been linked to neurodevelopmental disorders such as intellectual disability and microcephaly.

Picture

Western Blot

WB result of METTL1 Recombinant Rabbit mAb
Primary antibody: METTL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Lane 5: Caco-2 whole cell lysate 20 µg
Lane 6: HaCaT whole cell lysate 20 µg
Lane 7: A431 whole cell lysate 20 µg
Lane 8: HCT 116 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 28, 31 kDa

WB result of METTL1 Recombinant Rabbit mAb
Primary antibody: METTL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 28, 31 kDa

WB result of METTL1 Recombinant Rabbit mAb
Primary antibody: METTL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Lane 3: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 37 kDa

WB result of METTL1 Recombinant Rabbit mAb
Primary antibody: METTL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 28, 35 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-METTL1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-METTL1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver cancer. Anti-METTL1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-METTL1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse liver. Anti-METTL1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-METTL1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.