WB result of MED4 Rabbit mAb
Primary antibody: MED4 Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 36 kDa
MED4 Recombinant Rabbit mAb (S-R258)
MED4 Recombinant Rabbit mAb (S-R258)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MED4 |
| Synonyms | Mediator of RNA polymerase II transcription subunit 4, Activator-recruited cofactor 36 kDa component (ARC36), Mediator complex subunit 4, TRAP/SMCC/PC2 subunit p36 subunit, Vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36), ARC36, DRIP36, VDRIP, HSPC126 |
| Location | Nucleus |
| Accession | Q9NPJ6 |
| Clone Number | S-R258 |
| Antibody Type | Recombinant mAb |
| Application | WB, IP |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IP | 1:50 | null |
Background
Mediator of RNA polymerase II transcription subunit 4 also known as mediator complex subunit 4 (MED4), a component of Mediator or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36) is a protein that in humans is encoded by the MED4 gene. The protein encoded by this gene is a component of the vitamin D receptor-interacting protein (DRIP) complex which functions as a nuclear receptor coactivator. The DRIP complex is capable of activating nuclear receptors in a ligand-dependent manner.
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Western Blot
WB result of MED4 Rabbit mAb
Primary antibody: MED4 Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 36 kDa
WB result of MED4 Rabbit mAb
Primary antibody: MED4 Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 36 kDa
IP
MED4 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating MED4 in 0.4 mg HepG2 whole cell lysate.
Western blot was performed on the immunoprecipitate using MED4 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: HepG2 whole cell lysate 20 µg (Input)
Lane 2: MED4 Rabbit mAb IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HepG2 whole cell lysate
Predicted MW: 30 kDa
Observed MW: 36 kDa
（This blot was developed with high sensitivity substrate）
