WB result of MCT1/Monocarboxylic acid transporter 1 Recombinant Rabbit mAb
Primary antibody: MCT1/Monocarboxylic acid transporter 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled Raji whole cell lysate 20 µg
Lane 2: unboiled A431 whole cell lysate 20 µg
Lane 3: unboiled HepG2 whole cell lysate 20 µg
Lane 4: unboiled HL-60 whole cell lysate 20 µg
Lane 5: unboiled HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 46 kDa
MCT1/Monocarboxylic acid transporter 1 Recombinant Rabbit mAb (S-5144)
MCT1/Monocarboxylic acid transporter 1 Recombinant Rabbit mAb (S-5144)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MCT1/Monocarboxylic acid transporter 1 |
| Synonyms | Monocarboxylate transporter 1; Solute carrier family 16 member 1; SLC16A1 |
| Location | Cell membrane |
| Accession | P53985 |
| Clone Number | S-5144 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | Raji, A431, HepG2, HL-60, HeLa |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
Monocarboxylate transporter 1 (MCT1), also known as SLC16A1, is a proton-linked membrane transport protein belonging to the solute carrier 16 family that facilitates the bidirectional transport of monocarboxylates, such as lactate, pyruvate, and ketone bodies, across cell membranes in a pH-dependent manner. Widely expressed in various tissues including skeletal muscle, cardiac muscle, erythrocytes, and the blood-brain barrier, MCT1 plays a pivotal role in cellular energy metabolism by enabling the "lactate shuttle," a mechanism where lactate produced by glycolytic cells is transported into oxidative cells to be used as a fuel source for ATP production via the tricarboxylic acid (TCA) cycle. Its function is strictly coupled with the movement of protons, meaning that transport efficiency is heavily influenced by the transmembrane pH gradient, and it typically requires association with chaperone proteins like CD147 (basigin) or GP70 for proper trafficking to the plasma membrane and stability. Due to its critical involvement in metabolic flexibility, MCT1 is extensively studied in contexts ranging from athletic performance and ischemic heart disease to cancer metabolism, where tumor cells often exploit MCT1-mediated lactate uptake to support their high energetic demands and maintain redox balance, making it a potential therapeutic target for metabolic disorders and oncology.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-MCT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-MCT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human pancreas. Anti-MCT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-MCT1/Monocarboxylic acid transporter 1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
