1 1

MARCKS Recombinant Rabbit mAb (S-3190-72)

MARCKS Recombinant Rabbit mAb (S-3190-72)

Catalog Number: S0B6544 Application: WB, IHC-P, ICC Reactivity: Human Conjugation: Unconjugated Brand: Starter
Share:
Price:
$100 USD
$100 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Host Rabbit
Antigen MARCKS
Synonyms Myristoylated alanine-rich C-kinase substrate; Protein kinase C substrate; 80 kDa protein; light chain (80K-L protein; PKCSL); MACS; PRKCSL
Immunogen Synthetic Peptide
Location Cytoplasm, Cytoskeleton, Cell membrane
Accession P29966
Clone Number S-3190-72
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P, ICC
Reactivity Hu
Positive Sample A431, HEK-293
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Hu
IHC-P 1:500 Hu
ICC 1:500 Hu

Background

MARCKS (Myristoylated Alanine-Rich C Kinase Substrate) is an evolutionarily conserved, intrinsically disordered peripheral membrane protein that serves as a key phosphorylation substrate for conventional and novel protein kinase C isoforms; by reversibly sequestering phosphatidylinositol-4,5-bisphosphate (PIP2) and cross-linking actin filaments, it integrates PKC, Ca2+/calmodulin, and small GTPase signaling to orchestrate dynamic membrane-cytoskeleton remodeling underlying cell adhesion, migration, endo/exocytosis, neurotransmitter release, and stem-cell differentiation, while its dysregulation contributes to cancer metastasis, inflammatory diseases, and neuronal disorders.

Picture

Western Blot

WB result of MARCKS Recombinant Rabbit mAb
Primary antibody: MARCKS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 80 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-MARCKS antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-MARCKS antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-MARCKS antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-MARCKS antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in A431 cells (top panel) and negative staining in Jurkat cells (below panel). Anti-MARCKS antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).