WB result of MAP3K1 Recombinant Rabbit mAb
Primary antibody: MAP3K1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 164 kDa
Observed MW: 164 kDa
MAP3K1 Recombinant Rabbit mAb (S-3097-61)
MAP3K1 Recombinant Rabbit mAb (S-3097-61)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MAP3K1 |
| Synonyms | Mitogen-activated protein kinase kinase kinase 1; MAPK/ERK kinase kinase 1 (MEK kinase 1; MEKK 1); MAPKKK1; MEKK; MEKK1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q13233 |
| Clone Number | S-3097-61 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | 293T, SH-SY5Y, A431, A549, EL4, NIH/3T3, mouse testis, mouse embryo, rat testis, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
MAP3K1 (MEKK1) is a 196-kDa serine/threonine kinase that sits at the hub of multiple signaling cascades: it functions as the apical MAP3K in the classical ERK5 and JNK pathways, phosphorylating and activating MKK5 and MKK4/7 respectively, while also cross-talking to the canonical NF-κB axis by catalyzing IKKβ phosphorylation; beyond kinase activity it acts as a ubiquitin E3 ligase that targets downstream adaptors such as TRAF2 and DUSP1 for degradation, thereby fine-tuning stress, cytokine and receptor-tyrosine-kinase responses; gain-of-function missense mutations clustered in its N-terminal PH domain and C-terminal kinase domain are among the most frequent genomic events in estrogen-receptor-positive breast and endometrial cancers, where they enhance transcription of ER-dependent proliferative genes, drive anti-apoptotic signaling and confer resistance to endocrine therapies, making MAP3K1 both a key integrator of cellular stress decisions and an actionable driver of luminal-type women’s cancers.
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Western Blot
WB result of MAP3K1 Recombinant Rabbit mAb
Primary antibody: MAP3K1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: EL4 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: mouse testis lysate 20 µg
Lane 4: mouse embryo lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 164 kDa
Observed MW: 164 kDa
WB result of MAP3K1 Recombinant Rabbit mAb
Primary antibody: MAP3K1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 164 kDa
Observed MW: 164 kDa
WB result of MAP3K1 Recombinant Rabbit mAb
Primary antibody: MAP3K1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 164 kDa
Observed MW: 164 kDa
