WB result of MANF Recombinant Rabbit mAb
Primary antibody: MANF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 20 kDa
MANF Recombinant Rabbit mAb (S-4434-31)
MANF Recombinant Rabbit mAb (S-4434-31)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MANF |
| Synonyms | Mesencephalic astrocyte-derived neurotrophic factor; Arginine-rich protein; Protein ARMET; ARMET; ARP |
| Immunogen | Recombinant Protein |
| Location | Secreted, Endoplasmic reticulum |
| Accession | P55145 |
| Clone Number | S-4434-31 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | SH-SY5Y, NIH/3T3, mouse brain, rat brain |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:800-1:4000 | Hu, Ms, Rt |
| IF | 1:4000 | Ms, Rt |
Background
MANF (mesencephalic astrocyte-derived neurotrophic factor, also known as ARMET) is an evolutionarily highly conserved secreted protein, named after its initial isolation from rat midbrain astrocyte-conditioned medium and its discovery as a factor that promotes the survival of dopaminergic neurons; however, both its structure and mode of action differ from those of classical neurotrophic factors. Human MANF consists of 179 amino acids and contains an N-terminal saposin-like domain and a C-terminal SAP domain. Under normal conditions it resides mainly in the endoplasmic reticulum (ER) lumen, where it is retained through binding to BiP/HSPA5; when ER stress or hypoxia occurs, MANF expression is upregulated and the protein is secreted extracellularly. Mechanistically, MANF is a key negative regulator of the unfolded protein response (UPR): it can directly bind the ER transmembrane sensor IRE1α, reducing its oligomerization and suppressing downstream signaling such as XBP1 splicing, thereby protecting neurons from ER stress-induced death—an interaction essential for its neuroprotective effects in animal models of Parkinson's disease. Beyond the central nervous system, MANF exerts protective effects on pancreatic β cells, cardiomyocytes, retinal photoreceptors, and cells of various tissues including the liver and kidney, and MANF knockout mice develop a severe diabetic phenotype due to progressive β-cell loss. Recent studies have further revealed that after ischemic stroke, MANF expression shifts from neurons to activated microglia/macrophages, and recombinant MANF delivered intranasally or intravenously can reduce infarct volume and modulate inflammatory responses, suggesting its therapeutic potential as a broad-spectrum stress-protective and immunomodulatory factor.
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Western Blot
WB result of MANF Recombinant Rabbit mAb
Primary antibody: MANF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 20 kDa
WB result of MANF Recombinant Rabbit mAb
Primary antibody: MANF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Low expression control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 20 kDa
WB result of MANF Recombinant Rabbit mAb
Primary antibody: MANF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 20 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human brain. Anti-MANF antibody was used at 1/800 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows weak staining in paraffin-embedded human skeletal muscle. Anti-MANF antibody was used at 1/800 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse brain. Anti-MANF antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows weak staining in paraffin-embedded mouse skeletal muscle. Anti-MANF antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat brain. Anti-MANF antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows weak staining in paraffin-embedded rat skeletal muscle. Anti-MANF antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse brain. Anti-MANF antibody was used at 1/4000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat brain. Anti-MANF antibody was used at 1/4000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows negative staining in paraffin-embedded rat skeletal muscle. Anti-MANF antibody was used at 1/4000 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
