WB result of S-RMabMix™ Malonyl Lysine Rabbit mAb
Primary antibody: S-RMabMix™ Malonyl Lysine Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
This blot was developed with high sensitivity substrate
S-RMabMix™ Malonyl Lysine Rabbit mAb
S-RMabMix™ Malonyl Lysine Rabbit mAb
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Product Details
Product Details
Product Specification
| Synonyms | Malonylation; Kmal; Malonylation |
| Immunogen | Synthetic Peptide |
| Antibody Type | Recombinant mAb Mix |
| Isotype | IgG |
| Application | WB |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:2000 | Species Independent |
| WB | 1:1000 | Species Independent |
Background
Lysine malonylation (Kmal) is a dynamic and evolutionarily conserved post-translational modification in which a malonyl group, derived from the donor substrate malonyl-CoA, is covalently attached to the ε-amino group of a lysine residue. This modification dramatically alters the protein's physicochemical properties by shifting the net charge of the lysine residue from positive (+1) to negative (−1) at physiological pH, which can induce significant conformational changes and disrupt electrostatic interactions. Primarily regulated by the demalonylase SIRT5 and the acetyltransferase KAT2A, Kmal plays a crucial role in fine-tuning cellular metabolism, immune responses, and cancer progression by modifying key enzymes involved in glycolysis, fatty acid oxidation, and mitochondrial function.
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Western Blot
WB result of S-RMabMix™ Malonyl Lysine Rabbit mAb
Primary antibody: S-RMabMix™ Pyruvoyl Lysine Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
Dot Blot
Dot blot result of S-RMabMix™ Malonyl Lysine Rabbit mAb
Lane 1: Modified peptide library (Malonyl lysine)
Lane 2: Unmodified peptide library control
Lane 3: Modified peptide library (MethMalonyl lysine)
Lane 4: Modified peptide library (Fumaryl lysine)
Lane 5: Modified peptide library (Crotonyl lysine)
Lane 6: Modified peptide library (Succinyl lysine)
Lane 7: Modified peptide library (Butyryl lysine)
Lane 8: Modified peptide library (Propionyl lysine)
Lane 9: Modified peptide library (Actyl lysine)
Primary antibody: S-RMabMix™ Malonyl Lysine Rabbit mAb at 1/2000 dilution
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
