WB result of MAGE-A1 Rabbit mAb Primary antibody: MAGE-A1 Rabbit mAb at 1/5000 dilution Lane 1: A375 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 34 kDa Observed MW: 42 kDa
MAGE-A1 Recombinant Rabbit mAb (S-R183)
MAGE-A1 Recombinant Rabbit mAb (S-R183)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MAGE-A1 |
| Synonyms | Melanoma-associated antigen 1, Antigen MZ2-E, Cancer/testis antigen 1.1 (CT1.1), MAGE-1 antigen, MAGEA1, Cancer/testis antigens (CTAs) |
| Location | Cytoplasm, Nucleus |
| Accession | P43355 |
| Clone Number | S-R183 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000 | null |
| IHC | 1:2000 | null |
| ICC | 1:50 | null |
Background
Melanoma-associated antigen 1, also known as Cancer/testis antigens (CTAs), is a protein that in humans is encoded by the MAGEA1 gene [PMID: 1840703]. Cancer/testis antigens (CTAs) are expressed in several cancers and during normal adult male germ cell differentiation [PMID: 17208940].
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-MAGE-A1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-MAGE-A1 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
