WB result of MACIR Rabbit pAb
Primary antibody: MACIR Rabbit pAb at 1/1000 dilution
Lane 1: 293T transfected with empty vector whole cell lysate 20 µg
Lane 2: 293T transfected with MACIR -Myc-His fusion protein whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 30 kDa
MACIR Rabbit Polyclonal Antibody
MACIR Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MACIR |
| Synonyms | Macrophage immunometabolism regulator; C5orf30 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q96GV9 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
Background
MACIR (macrophage immunometabolism regulator), previously known as C5orf30, is a protein that plays a crucial role in regulating macrophage function and has potential applications in treating chronic inflammation. It is a 206 amino acid protein with no significant similarity to any other human protein. MACIR is highly conserved in vertebrate genomes and is expressed in various tissues, including the colon and brain. It functions as a negative regulator of tissue damage and modulates the activity of synovial fibroblasts and macrophages, which are associated with rheumatoid arthritis. MACIR enhances the resolution of inflammation and wound repair functions mediated by M2 macrophages by inhibiting glycolysis. Additionally, it may play a role in protein trafficking through its interaction with UNC119 and UNC119B cargo adapters and in ciliary membrane localization via its interaction with UNC119B.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-MACIR antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-MACIR antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-MACIR antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
