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M-CSF Protein, Mouse

M-CSF Protein, Mouse

Catalog Number: UA040583 Brand: UA BIOSCIENCE
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$120 USD
$120 USD
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Product Details

Product Specification


Species Mouse
Synonyms M-CSF, CSF-1, Macrophage Colony Stimulating Factor
Accession P07141
Amino Acid Sequence

Lys33-Glu262
Expression System HEK293
Molecular Weight

45-60 kDa (Reducing)
Purity >95% by SDS-PAGE.
Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tag No Tag
Physical Appearance Lyophilized Powder
Storage Buffer

PBS, pH7.4
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.
Reference

Behring Inst Mitt. 1988 Aug:(83):15-26.

Background

Macrophage-Colony Stimulating Factor (M-CSF), also known as Colony Stimulating Factor-1 (CSF-1), is a hematopoietic growth factor. It can stimulate the survival, proliferation and differentiation of mononuclear phagocytes, in addition to the spreading and motility of macrophages. M-CSF is mainly produced by monocytes, macrophages, fibroblasts, and endothelial cells. M-CSF interaction with its receptor, c-fms, has been implicated in the growth, invasion, and metastasis of several diseases, including breast and endometrial cancers.

Picture

Bioactivity

Upper*: Representative images of bone marrow-derived macrophages (BMDM).
2e5 cells/ml BMDM seeding density, 10 ng/ml M-CSF induction for 24h, 48h, 96h, 120h, respectively.
Lower: Over 99% of the cells were positive for CD11b after 120h induction.
*1. Less attached cell is observed after 24h induction, and continuously culture is recommended, when the seeding density is less than 1e5.
*2. Higher cell density is observed in 24-well plate than 6-well plate, when the same seeding density is plated.

SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).

ELISA

Immobilized M-CSF Protein, Mouse (Cat. No. UA040583) at 2.0μg/mL (100μL/well) can bind M-CSFR mFc Chimera, Mouse (Cat. No. UA010188) with EC50 of 4.3-6.4 ng/ ml.