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M-CSF Protein, Mouse

M-CSF Protein, Mouse

Catalog Number: UA040128 Brand: UA BIOSCIENCE
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$144 USD
$144 USD
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Product Details

Product Specification


Species Mouse
Synonyms M-CSF,CSF-1,Macrophage Colony Stimulating Factor
Accession P07141
Amino Acid Sequence

Lys33-Glu262
Expression System HEK293
Molecular Weight

26 KDa
Purity ＞95% by SDS-PAGE
Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tag No Tag
Physical Appearance Lyophilized Powder
Storage Buffer

PBS, pH 7.4. 
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

· 3 months, -20 to -80℃ under sterile conditions after reconstitution.

· 1 week, 2 to 8℃ under sterile conditions after reconstitution.

· Please avoid repeated freeze-thaw cycles.
Reference

1. Cosman D, Wignall J, Anderson D, etal. 1988. Behring Inst Mitt: 15-26.

Background

Macrophage-Colony Stimulating Factor (M-CSF),also known as Colony Stimulating Factor-1 (CSF-1), is a hematopoietic growthfactor. It can stimulate the survival, proliferation and differentiation ofmononuclear phagocytes, in addition to the spreading and motility ofmacrophages. M-CSF is mainly produced by monocytes, macrophages, fibroblasts,and endothelial cells. M-CSF interaction with its receptor, c-fms, has beenimplicated in the growth, invasion, and metastasis of several diseases,including breast and endometrial cancers.

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Bioactivity

Measured in a cell proliferation assay using Murine M-NFS-60 cells, the EC50 for this effect is less than 10ng/ml.

SDS-PAGE

1μg (R: reducing condition, N: non-reducing condition).

ELISA

Immobilized M-CSF Protein, Mouse (Cat. No. UA040128) at 2.0μg/mL (100μL/well) can bind M-CSFR mFc Chimera, Mouse(Cat. No. UA010188) with EC50 of 5.52-8.06 ng/ mL.