M-CSF Protein, Human
M-CSF Protein, Human
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | M-CSF, CSF-1, Lanimostim, Macrophage Colony Stimulating Factor |
| Accession | P09603-3 |
| Amino Acid Sequence | Glu33-Ser190 |
| Expression System | E.coli |
| Molecular Weight | 19kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 50mM Tris, 300mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1、Sullivan A R. et al. (2014) CSF-1R Signaling in Health and Disease: A Focus on the Mammary Gland. J Mammary Gland Biol Neoplasia. 19: 149-159. 2、Griffin J D. et al. (1990) The biology of GM-CSF: regulation of production and interaction with its receptor. Int J Cell Cloning. S1: 35-44. |
Background
Macrophage-Colony Stimulating Factor (M-CSF), also known as Colony Stimulating Factor-1 (CSF-1), is a hematopoietic growth factor. It can stimulate the survival, proliferation and differentiation of mononuclear phagocytes, in addition to the spreading and motility of macrophages. M-CSF is mainly produced by monocytes, macrophages, fibroblasts, and endothelial cells. M-CSF interaction with its receptor, c-fms, has been implicated in the growth, invasion, and metastasis of of several diseases, including breast and endometrial cancers. The biological activity of human M-CSF is only active in the disulfide-linked dimeric form, which is bonded at Cys63.
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Bioactivity
SDS-PAGE
ELISA
Immobilized M-CSF Protein, Human (Cat. No. UA040042) at 0.5μg/mL (100μL/well) can bind M-CSFR Fc Chimera Protein, Human (Cat. No. UA010186) with EC50 of 33.09-51.56ng/mL.
SPR
Anti-His antibody Immobilized on CM5 Chip captured M-CSFR His Tag, Human (Cat. No. UA010208), can bind M-CSF, Human (Cat. No. UA040042) with an affinity constant of 10.76nM as determined in SPR assay.
Anti-His antibody Immobilized on CM5 Chip captured M-CSFR His Tag, Cynomolgus (Cat. No. UA010204), can bind M-CSF, Human (Cat. No. UA040042) with an affinity constant of 0.3μM as determined in SPR assay.
